Functional assays in prostate carcinoma cell lines

Coordinator:    Prof. Dr. Jan Mollenhauer
Institution: University of Southern Denmark
In the last years, various molecular markers of prostate cancer initiation and progression have been identified on both DNA and protein level. Functional information is essential to fully understand the contribution of the identified molecules to prostate cancer pathophysiology, yet it is still missing. The lack of suitable methods is probably the major reason for the considerable gap between the description of genomic, transcriptomic and proteomic profiles and the phenotype they might determine.
The goal of this subproject is to bridge the gap by employing a highly standardized cellular system for the functional analysis of genes identified in subprojects 2-6. At first, acceptor cell lines, which readily accept introduction of genes via site-specific recombination, are being generated from well-established prostate cancer in vitro models (PC-3, LNCaP). This will be followed by construction of an isogenic cell line library, in which each clone stably overexpresses one candidate gene
After an initial screen of the library to identify novel genes that influence cancer cell growth viability, other parameters like invasion and migration potential of cells will be determined. Based on these results, selected cell lines will be analyzed in vivo using the nude mouse xenograft model (SP12). Ultimately, the effort should yield novel, well-characterized genes that might serve as future drug targets for the therapy prostate cancer.
INTRANET (Members login)