Molecular Phenotyping Screen

Coordinator:		PD Dr. Johannes Beckers
Institution:		Institut für Experimentelle Genetik, Helmholtz Zentrum München
Homepage:		www.helmholtz-muenchen.de/en/ieg

The goals of the molecular phenotyping screen are the routine analysis of genome-wide RNA expression profiles of organs from mouse models and the molecular description of models for human diseases. For standardized expression profiling analysis we currently use commercial, whole-genome Illumina Bead arrays with about 44.000 probes. In the primary screen, a set of eight organs (spleen, kidney, liver, heart, thymus, skeletal muscle, brain, and pancreas) from mutant and control mice is systematically collected for each mutant mouse line. Samples for gene expression profiling are selected base on conspicuous phenotypes in other GMC screens or on previous knowledge of predicted gene function or affected organs. In the secondary screen tissues that are not included in the routine set of organs or that are collected at a different age, from different sexes or after environmental challenges are analyzed. Statistical analysis is performed for the identification of significant differences in gene expression.



Additional relevant Internet links:
The German Mouse Clinic