Production of protein-tagged pluripotent und differentiated ES cells

Coordinator:		Prof. Dr. Wolfgang Wurst
Institution:		Institut für Entwicklungsgenetik, Helmholtz Zentrum München
Homepage:		www.helmholtz-muenchen.de

In subproject 3 the disease genes marked with fluorescence dyes by TP1 are introduced into murine embryonal stem cells (ES cells) via electroporation. During the project duration the applied methods have been constantly improved and refined. Therefore over 200 ES cell lines could be generated which express the proteins marked with a fluorescent dye – in the meantime with a success rate of over 60 percent. All these cell lines were forwarded to SP6 to identify the protein interaction partners of tagged proteins using mass spectrometry and thus determine the core proteome of these disease candidate genes. Species specific interaction partners of disease genes can be determined by carrying out a comparative analysis with human ES cell lines in which the same proteins were tagged. All cell lines produced in subproject 3 have been stored and are available to the whole scientific community.


Additional relevant Internet links:
DiGTOP