NGFN-PLUS

Production and imaging of HeLa and ES cell lines

Coordinator:    Prof. Dr. Tony Hyman
Institution: Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)
Homepage: www.mpi-cbg.de/research
The goal of this project is to develop and use standardized pipelines for producing HeLa cells and ES cells with tagged transgenes that allow visualization of the transgene to determine its localization and immunoprecipitation to determine the protein complexes. The second goal of this subproject is to establish standardized ways of imaging ES cells containing the transgenes. This will allow a validation of the transgene, and provide a source of cells for immunoprecipitation by SP6. The results generated by this subproject will allow to localize cellular proteins associated with human diseases. Thus, this work forms the basis of a system biological approach in order to characterise disease genes.




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