Systematic phenotype analysis using RNAi

Coordinator:		Prof. Dr. Michael Boutros
Institution:		DKFZ Heidelberg
Homepage:		www.dkfz.de/signaling/

Our research goal is a systems-level approach to analyse signalling pathways playing 
an important role in neurodegenerative diseases. In order to identify functional 
modules, we will systematically examine these pathways using gene-phenotype profiles. 
This subproject will focus on signalling pathways directly relevant to 
neurodegenerative diseases. In a first step, we will establish and optimise cell based 
assays to identify new targets by RNAi screenings. Another goal of the project is the 
identification of new small molecules selectively inhibiting signalling pathways. 
Using bioinformatic tools, these data will be integrated with protein-protein 
interactions to identify disease specific targets and new signalling pathway modules.
The identification of new targets and their connection to signalling networks are 
highly important for the development of new therapeutic approaches.

                 
   
                                

RNAi Screening:
Cell-based high-throughput RNAi screens are performed in 384-well plate formats. Shown is a single readout and signal intensities refer to cell viability of cells treated with RNAi molecules targeting specific genes.