Mouse models for the in vivo validation of protein interactions

Coordinator:		Dr. Ralf Kühn
Institution:		Institut für Entwicklungsgenetik, Helmholtz Zentrum München
Homepage:		www.helmholtz-muenchen.de/idg

SP8 will generate mouse models that express tagged proteins in vivo.

These mouse models will be used:

i) as demonstration activities for the utilization of the DiGTOP protein tagged ES cell line resource in mice;
ii) to validate observations made in the cellular models by recapitulating them in the mouse;
iii) to explore the application of protein tag multi-purpose alleles and iv) to access specific cell types in the physiological context.

The set of ~ 30 key disease genes examined and validated by this subproject will be selected because the protein interaction mapping  provides the data for choice. Those proteins which show multiple interaction partners in vitro in different cell types  will be chosen. The generated mouse models will be used to study altered protein pathways by iterative analysis of the interaction profile in a cell type-specific fashion.
Therefore, this SP will play a central role in DiGTOP by in vivo validation of protein interaction profile and the provision of animal models to the DiGTOP consortium and to our collaboration partners within the framework of NGFNplus.


Additional relevant Internet links:
DiGTOP