Validation and pathway dissection of disease genes using endoribonucelase prepared siRNAs

Coordinator:		Dr. Frank Buchholz
Institution:		Max Planck Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)
Homepage:		www.mpi-cbg.de/research/research-groups/frank-buchholz.html

The research goal of this subproject is to provide functional data for the protein-protein interaction data through loss-of-function studies. This functional data will be obtained through RNA interference (RNAi) studies employing the highly efficient and specific endoribonuclease prepared (e)siRNA resource developed within NGFN2.

The functional characterization will be performed on three levels:

1.    Knockdown of candidate proteins applying existing and novel assays to characterize phenotypic consequences.
2.    Determining the protein complex assembly by consecutive knockdowns in reporter cell lines generated for all protein complex components.
3.    Large-scale knockdowns to study the global mRNA and protein landscape after depletion of candidate proteins.
This functional dissection of the studied protein networks will also be very valuable to judge whether the protein complex- and which proteins in the complex are promising candidates for drug development.

Additional relevant Internet links:
DiGTOP