Quantitative Proteomics

Coordinator:		PD Dr. Gerard Drewes
Institution:		Cellzome AG
Homepage:		www.cellzome.com

The goal of this sub-project is to elucidate the mechanisms by which mutations in proteins in tumours cause a de-regulation of their associated protein complexes. To this goal, a selection of the mutant proteins studied by the IG-Mutanom consortium will be subjected to quantitative interactome analysis. In the first step, protein tagging and affinity chromatography will be employed to isolate native protein complexes formed by the mutated and wild-type proteins from cultured tumour cell lines. In the next step, we will then use state-of-the-art quantitative mass spectrometry to map all proteins in the purified protein complex and to quantify their relative amounts. By directly comparing the data obtained for wildtype and mutated proteins, differences in the stoichiometry of the complexes will be calculated. Comparative quantitative proteomics profiling of cancer model cell lines will also be performed. From these data, the molecular basis of the abnormal signal transduction in the tumour can be deduced. Together with additional orthogonal data generated by IG-Mutanom, this should enable the establishment of immunoaffinity-based analysis methods for tumour biopsies with the final goal of developing assays for diagnostic and compound screening purposes.


Additional relevant Internet link:
The Mutanom project